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Precisely what is the maximum relative centrifugal power applied when red blood cells are sedimented at 1000 rpm inside a rotor of optimum sample radius equal to 10 cm?
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Differential centrifugation has numerous important disadvantages. Because the homogenate is to begin with distributed uniformly throughout the centrifuge tube, the 1st particles sedimented will always be contaminated with compact amounts of all other constituents of your homogenate. This impact is demonstrated in Determine 12-5.
However, significant complications crop up When the particles to get divided have identical sedimentation coefficients. This problem is website illustrated in Figure 12-4f, during which the mitochondrial portion also has the cells’ lysosomes.